CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity

Hong-Gu Kang (), Hyong Woo Choi, Sabrina von Einem, Patricia Manosalva, Katrin Ehlers, Po-Pu Liu, Stefanie V. Buxa, Magali Moreau, Hyung-Gon Mang, Pradeep Kachroo, Karl-Heinz Kogel and Daniel F. Klessig ()
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Hong-Gu Kang: Boyce Thompson Institute for Plant Research
Hyong Woo Choi: Boyce Thompson Institute for Plant Research
Sabrina von Einem: Research Center for Bio Systems, Land Use, and Nutrition
Patricia Manosalva: Boyce Thompson Institute for Plant Research
Katrin Ehlers: Research Center for Bio Systems, Land Use, and Nutrition
Po-Pu Liu: Boyce Thompson Institute for Plant Research
Stefanie V. Buxa: Research Center for Bio Systems, Land Use, and Nutrition
Magali Moreau: Boyce Thompson Institute for Plant Research
Hyung-Gon Mang: Texas State University
Pradeep Kachroo: University of Kentucky
Karl-Heinz Kogel: Research Center for Bio Systems, Land Use, and Nutrition
Daniel F. Klessig: Boyce Thompson Institute for Plant Research

Nature Communications, 2012, vol. 3, issue 1, 1-11

Abstract: Abstract Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1, are also required for pathogen-associated molecular pattern (PAMP)-triggered immunity, basal resistance, non-host resistance and systemic acquired resistance. Consistent with its role in PAMP-triggered immunity, CRT1 interacted with the PAMP recognition receptor FLS2. Subcellular fractionation and transmission electron microscopy detected a subpopulation of CRT1 in the nucleus, whose levels increased following PAMP treatment or infection with an avirulent pathogen. These results, combined with the demonstration that CRT1 binds DNA, exhibits endonuclease activity, and affects tolerance to the DNA-damaging agent mitomycin C, argue that this prototypic eukaryotic member of the MORC superfamily has important nuclear functions during immune response activation.

Date: 2012
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DOI: 10.1038/ncomms2279

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