Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells

Shin-Ichi Mae, Akemi Shono, Fumihiko Shiota, Tetsuhiko Yasuno, Masatoshi Kajiwara, Nanaka Gotoda-Nishimura, Sayaka Arai, Aiko Sato-Otubo, Taro Toyoda, Kazutoshi Takahashi, Naoki Nakayama, Chad A. Cowan, Takashi Aoi, Seishi Ogawa, Andrew P. McMahon, Shinya Yamanaka and Kenji Osafune ()
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Shin-Ichi Mae: Center for iPS Cell Research and Application, Kyoto University
Akemi Shono: Center for iPS Cell Research and Application, Kyoto University
Fumihiko Shiota: Center for iPS Cell Research and Application, Kyoto University
Tetsuhiko Yasuno: Center for iPS Cell Research and Application, Kyoto University
Masatoshi Kajiwara: Center for iPS Cell Research and Application, Kyoto University
Nanaka Gotoda-Nishimura: Center for iPS Cell Research and Application, Kyoto University
Sayaka Arai: Center for iPS Cell Research and Application, Kyoto University
Aiko Sato-Otubo: Cancer Genomics Project, The University of Tokyo
Taro Toyoda: Center for iPS Cell Research and Application, Kyoto University
Kazutoshi Takahashi: Center for iPS Cell Research and Application, Kyoto University
Naoki Nakayama: Centre for Stem Cell Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston
Chad A. Cowan: Harvard Stem Cell Institute
Takashi Aoi: Center for iPS Cell Research and Application, Kyoto University
Seishi Ogawa: Cancer Genomics Project, The University of Tokyo
Andrew P. McMahon: Eli and Edythe Broad-Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern California
Shinya Yamanaka: Center for iPS Cell Research and Application, Kyoto University
Kenji Osafune: Center for iPS Cell Research and Application, Kyoto University

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract A method for stimulating the differentiation of human pluripotent stem cells into kidney lineages remains to be developed. Most cells in kidney are derived from an embryonic germ layer known as intermediate mesoderm. Here we show the establishment of an efficient system of homologous recombination in human pluripotent stem cells by means of bacterial artificial chromosome-based vectors and single-nucleotide polymorphism array-based detection. This system allowed us to generate human-induced pluripotent stem cell lines containing green fluorescence protein knocked into OSR1, a specific intermediate mesoderm marker. We have also established a robust induction protocol for intermediate mesoderm, which produces up to 90% OSR1+ cells. These human intermediate mesoderm cells can differentiate into multiple cell types of intermediate mesoderm-derived organs in vitro and in vivo, thereby supplying a useful system to elucidate the mechanisms of intermediate mesoderm development and potentially providing a cell source for regenerative therapies of the kidney.

Date: 2013
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DOI: 10.1038/ncomms2378

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