Synthetic RNA devices to expedite the evolution of metabolite-producing microbes

Jina Yang, Sang Woo Seo, Sungho Jang, So-I Shin, Chae Hyun Lim, Tae-Young Roh and Gyoo Yeol Jung ()
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Jina Yang: Pohang University of Science and Technology
Sang Woo Seo: Pohang University of Science and Technology
Sungho Jang: Pohang University of Science and Technology
So-I Shin: Pohang University of Science and Technology
Chae Hyun Lim: Pohang University of Science and Technology
Tae-Young Roh: Pohang University of Science and Technology
Gyoo Yeol Jung: Pohang University of Science and Technology

Nature Communications, 2013, vol. 4, issue 1, 1-7

Abstract: Abstract An extension of directed evolution strategies to genome-wide variations increases the chance of obtaining metabolite-overproducing microbes. However, a general high-throughput screening platform for selecting improved strains remains out of reach. Here, to expedite the evolution of metabolite-producing microbes, we utilize synthetic RNA devices comprising a riboswitch and a selection module that specifically sense inconspicuous metabolites. Using L-lysine-producing Escherichia coli as a model system, we demonstrated that this RNA device could enrich pathway-optimized strains to up to 75% of the total population after four rounds of enrichment cycles. Furthermore, the potential applicability of this device was examined by successfully extending its application to the case of L-tryptophan. When used in conjunction with combinatorial mutagenesis for metabolite overproduction, our synthetic RNA device should facilitate strain improvement.

Date: 2013
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DOI: 10.1038/ncomms2404

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