Actin-based confinement of calcium responses during Shigella invasion

Guy Tran Van Nhieu (), Bing Kai Liu, Jie Zhang, Fabienne Pierre, Sylvie Prigent, Philippe Sansonetti, Christophe Erneux, Jung Kuk Kim, Pann-Ghill Suh, Geneviève Dupont and Laurent Combettes
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Guy Tran Van Nhieu: Equipe Communication Intercellulaire et Infections Microbiennes. Centre de Recherche Interdisciplinaire en Biologie (CIRB). Collège de France
Bing Kai Liu: Equipe Communication Intercellulaire et Infections Microbiennes. Centre de Recherche Interdisciplinaire en Biologie (CIRB). Collège de France
Jie Zhang: Equipe Communication Intercellulaire et Infections Microbiennes. Centre de Recherche Interdisciplinaire en Biologie (CIRB). Collège de France
Fabienne Pierre: Equipe Communication Intercellulaire et Infections Microbiennes. Centre de Recherche Interdisciplinaire en Biologie (CIRB). Collège de France
Sylvie Prigent: Université Paris Sud, UMRS757
Philippe Sansonetti: Unité de Pathogénie Microbienne Moléculaire. Département de Biologie Cellulaire et Infections. Institut Pasteur
Christophe Erneux: Interdisciplinary Research Institute (IRIBHM), Université Libre de Bruxelles, Campus Erasme, Bldg C
Jung Kuk Kim: Pohang University of Science and Technology
Pann-Ghill Suh: Pohang University of Science and Technology
Geneviève Dupont: Unité de Chronobiologie Théorique. Université Libre de Bruxelles. CP231
Laurent Combettes: Université Paris Sud, UMRS757

Nature Communications, 2013, vol. 4, issue 1, 1-10

Abstract: Abstract Shigella, the agent of bacillary dysentery, invades epithelial cells by locally inducing actin reorganization. Upon cell invasion, Shigella induces calcium (Ca2+) signalling, but its role in invasion has remained unclear. Here we show that components involved in inositol 1, 4, 5- trisphosphate (InsP3) signalling are implicated in Shigella invasion. Although global Ca2+ responses are dispensable for bacterial invasion, local Ca2+ responses of unprecedented long duration are associated with invasion sites. Fluorescence recovery after photo-bleaching experiments indicate that diffusion of small solutes is hindered at Shigella-invasion sites and that diffusion hindrance is dependent on bacterially induced actin reorganization. Computational simulations and experimental challenge of the model support the notion that local accumulation of InsP3 permitted by restricted diffusion and enrichment of InsP3 receptors account for sustained local Ca2+ increases at entry sites. Thus, cytoskeletal reorganization through diffusion hindrance shapes the duration of local Ca2+ signals.

Date: 2013
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DOI: 10.1038/ncomms2561

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