The role and assembly mechanism of nucleoprotein in influenza A virus ribonucleoprotein complexes

Lauren Turrell, Jon W. Lyall, Laurence S. Tiley, Ervin Fodor and Frank T. Vreede ()
Additional contact information
Lauren Turrell: Sir William Dunn School of Pathology, University of Oxford
Jon W. Lyall: University of Cambridge
Laurence S. Tiley: University of Cambridge
Ervin Fodor: Sir William Dunn School of Pathology, University of Oxford
Frank T. Vreede: Sir William Dunn School of Pathology, University of Oxford

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract The nucleoprotein of negative-strand RNA viruses forms a major component of the ribonucleoprotein complex that is responsible for viral transcription and replication. However, the precise role of nucleoprotein in viral RNA transcription and replication is not clear. Here we show that nucleoprotein of influenza A virus is entirely dispensable for replication and transcription of short viral RNA-like templates in vivo, suggesting that nucleoprotein represents an elongation factor for the viral RNA polymerase. We also find that the recruitment of nucleoprotein to nascent ribonucleoprotein complexes during replication of full-length viral genes is mediated through nucleoprotein–nucleoprotein homo-oligomerization in a ‘tail loop-first’ orientation and is independent of RNA binding. This work demonstrates that nucleoprotein does not regulate the initiation and termination of transcription and replication by the viral polymerase in vivo, and provides new mechanistic insights into the assembly and regulation of viral ribonucleoprotein complexes.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms2589 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2589

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms2589

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().