Magnetic barcode assay for genetic detection of pathogens

Monty Liong, Anh N. Hoang, Jaehoon Chung, Nil Gural, Christopher B. Ford, Changwook Min, Rupal R. Shah, Rushdy Ahmad, Marta Fernandez-Suarez, Sarah M. Fortune, Mehmet Toner, Hakho Lee () and Ralph Weissleder ()
Additional contact information
Monty Liong: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School
Anh N. Hoang: BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School
Jaehoon Chung: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School
Nil Gural: BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School
Christopher B. Ford: Harvard School of Public Health, Boston, Massachusetts 02115, USA
Changwook Min: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School
Rupal R. Shah: Harvard School of Public Health, Boston, Massachusetts 02115, USA
Rushdy Ahmad: Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
Marta Fernandez-Suarez: BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School
Sarah M. Fortune: Harvard School of Public Health, Boston, Massachusetts 02115, USA
Mehmet Toner: BioMEMS Resource Center, Surgical Services, Massachusetts General Hospital, Harvard Medical School
Hakho Lee: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School
Ralph Weissleder: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School

Nature Communications, 2013, vol. 4, issue 1, 1-9

Abstract: Abstract The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms2745 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms2745

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms2745

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().