Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity

Edwin D. Hawkins, Jane Oliaro, Axel Kallies, Gabrielle T. Belz, Andrew Filby, Thea Hogan, Nicole Haynes, Kelly M. Ramsbottom, Vanessa Van Ham, Tanja Kinwell, Benedict Seddon, Derek Davies, David Tarlinton, Andrew M. Lew, Patrick O. Humbert and Sarah M. Russell ()
Additional contact information
Edwin D. Hawkins: Cancer Immunology Program, Peter MacCallum Cancer Centre
Jane Oliaro: Cancer Immunology Program, Peter MacCallum Cancer Centre
Axel Kallies: The Walter and Eliza Hall Institute of Medical Research
Gabrielle T. Belz: The Walter and Eliza Hall Institute of Medical Research
Andrew Filby: FACS Laboratory, London Research Institute, Cancer Research UK, 44 Lincolns Inn Fields
Thea Hogan: MRC National Institute for Medical Research, The Ridgeway, Mill Hill
Nicole Haynes: Cancer Immunology Program, Peter MacCallum Cancer Centre
Kelly M. Ramsbottom: Cancer Immunology Program, Peter MacCallum Cancer Centre
Vanessa Van Ham: Cancer Immunology Program, Peter MacCallum Cancer Centre
Tanja Kinwell: The University of Melbourne
Benedict Seddon: MRC National Institute for Medical Research, The Ridgeway, Mill Hill
Derek Davies: FACS Laboratory, London Research Institute, Cancer Research UK, 44 Lincolns Inn Fields
David Tarlinton: The Walter and Eliza Hall Institute of Medical Research
Andrew M. Lew: The Walter and Eliza Hall Institute of Medical Research
Patrick O. Humbert: The University of Melbourne
Sarah M. Russell: Cancer Immunology Program, Peter MacCallum Cancer Centre

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.

Date: 2013
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DOI: 10.1038/ncomms2796

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