Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qβ phage replicase

Nikita N. Vasilyev, Zarina S. Kutlubaeva, Victor I. Ugarov, Helena V. Chetverina and Alexander B. Chetverin ()
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Nikita N. Vasilyev: Institute of Protein Research of the Russian Academy of Sciences
Zarina S. Kutlubaeva: Institute of Protein Research of the Russian Academy of Sciences
Victor I. Ugarov: Institute of Protein Research of the Russian Academy of Sciences
Helena V. Chetverina: Institute of Protein Research of the Russian Academy of Sciences
Alexander B. Chetverin: Institute of Protein Research of the Russian Academy of Sciences

Nature Communications, 2013, vol. 4, issue 1, 1-7

Abstract: Abstract S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved in later events. Here, we show that in Qβ replicase-mediated RNA synthesis, S1 functions at the termination step by promoting release of the product strand in a single-stranded form. This function is fulfilled by the N-terminal fragment comprising the first two S1 domains. The results suggest that S1 might also have a role other than mRNA binding in the ribosome.

Date: 2013
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DOI: 10.1038/ncomms2807

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