Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control

James Dodgson, Anatole Chessel, Miki Yamamoto, Federico Vaggi, Susan Cox, Edward Rosten, David Albrecht, Marco Geymonat, Attila Csikasz-Nagy, Masamitsu Sato and Rafael E. Carazo-Salas ()
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James Dodgson: The Gurdon Institute, University of Cambridge
Anatole Chessel: The Gurdon Institute, University of Cambridge
Miki Yamamoto: University of Tokyo
Federico Vaggi: The Microsoft Research-University of Trento Centre for Computational Systems Biology, Piazza Manifattura 1, Rovereto 38068, Italy
Susan Cox: New Hunt's House, King’s College London
Edward Rosten: University of Cambridge
David Albrecht: Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, HPM G16.2, Zurich CH-8093, Switzerland
Marco Geymonat: The Gurdon Institute, University of Cambridge
Attila Csikasz-Nagy: The Microsoft Research-University of Trento Centre for Computational Systems Biology, Piazza Manifattura 1, Rovereto 38068, Italy
Masamitsu Sato: University of Tokyo
Rafael E. Carazo-Salas: The Gurdon Institute, University of Cambridge

Nature Communications, 2013, vol. 4, issue 1, 1-9

Abstract: Abstract Cell polarity is regulated by evolutionarily conserved polarity factors whose precise higher-order organization at the cell cortex is largely unknown. Here we image frontally the cortex of live fission yeast cells using time-lapse and super-resolution microscopy. Interestingly, we find that polarity factors are organized in discrete cortical clusters resolvable to ~50–100 nm in size, which can form and become cortically enriched by oligomerization. We show that forced co-localization of the polarity factors Tea1 and Tea3 results in polarity defects, suggesting that the maintenance of both factors in distinct clusters is required for polarity. However, during mitosis, their co-localization increases, and Tea3 helps to retain the cortical localization of the Tea1 growth landmark in preparation for growth reactivation following mitosis. Thus, regulated spatial segregation of polarity factor clusters provides a means to spatio-temporally control cell polarity at the cell cortex. We observe similar clusters in Saccharomyces cerevisiae and Caenorhabditis elegans cells, indicating this could be a universal regulatory feature.

Date: 2013
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DOI: 10.1038/ncomms2813

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