Picornavirus uncoating intermediate captured in atomic detail

Jingshan Ren, Xiangxi Wang, Zhongyu Hu, Qiang Gao, Yao Sun, Xuemei Li, Claudine Porta, Thomas S. Walter, Robert J. Gilbert, Yuguang Zhao, Danny Axford, Mark Williams, Katherine McAuley, David J. Rowlands, Weidong Yin, Junzhi Wang (), David I. Stuart (), Zihe Rao and Elizabeth E. Fry
Additional contact information
Jingshan Ren: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Xiangxi Wang: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science
Zhongyu Hu: National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Beijing 100050, China
Qiang Gao: Sinovac Biotech Co., Ltd
Yao Sun: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science
Xuemei Li: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science
Claudine Porta: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Thomas S. Walter: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Robert J. Gilbert: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Yuguang Zhao: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Danny Axford: Diamond Light Sources, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
Mark Williams: Diamond Light Sources, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
Katherine McAuley: Diamond Light Sources, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
David J. Rowlands: Faculty of Biological Sciences, Institute of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, University of Leeds
Weidong Yin: Sinovac Biotech Co., Ltd
Junzhi Wang: National Institutes for Food and Drug Control, No. 2, Tiantan Xili, Beijing 100050, China
David I. Stuart: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK
Zihe Rao: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science
Elizabeth E. Fry: University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK

Nature Communications, 2013, vol. 4, issue 1, 1-7

Abstract: Abstract It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release.

Date: 2013
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DOI: 10.1038/ncomms2889

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