Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis

Haiyun Gan, Lu Wen, Shangying Liao, Xiwen Lin, Tingting Ma, Jun Liu, Chun-xiao Song, Min Wang, Chuan He, Chunsheng Han () and Fuchou Tang ()
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Haiyun Gan: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Lu Wen: Biodynamic Optical Imaging Center, College of Life Sciences, Peking University
Shangying Liao: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Xiwen Lin: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Tingting Ma: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Jun Liu: College of Chemistry and Molecular Engineering, Peking University
Chun-xiao Song: The University of Chicago
Min Wang: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Chuan He: College of Chemistry and Molecular Engineering, Peking University
Chunsheng Han: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
Fuchou Tang: Biodynamic Optical Imaging Center, College of Life Sciences, Peking University

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract Little is known about how patterns of DNA methylation change during mammalian spermatogenesis. 5hmC has been recognized as a stable intermediate of DNA demethylation with potential regulatory functions in the mammalian genome. However, its global pattern in germ cells has yet to be addressed. Here, we first conducted absolute quantification of 5hmC in eight consecutive types of mouse spermatogenic cells using liquid chromatography-tandem mass spectrometry, and then mapped its distributions in various genomic regions using our chemical labeling and enrichment method coupled with deep sequencing. We found that 5hmC mapped differentially to and changed dynamically in genomic regions related to expression regulation of protein-coding genes, piRNA precursor genes and repetitive elements. Moreover, 5hmC content correlated with the levels of various transcripts quantified by RNA-seq. These results suggest that the highly ordered alterations of 5hmC in the mouse genome are potentially crucial for the differentiation of spermatogenic cells.

Date: 2013
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DOI: 10.1038/ncomms2995

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