Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

Satoru Okamoto, Hidefumi Shinohara, Tomoko Mori, Yoshikatsu Matsubayashi () and Masayoshi Kawaguchi ()
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Satoru Okamoto: National Institute for Basic Biology, Nishigonaka 38
Hidefumi Shinohara: National Institute for Basic Biology, Nishigonaka 38
Tomoko Mori: National Institute for Basic Biology, Nishigonaka 38
Yoshikatsu Matsubayashi: National Institute for Basic Biology, Nishigonaka 38
Masayoshi Kawaguchi: National Institute for Basic Biology, Nishigonaka 38

Nature Communications, 2013, vol. 4, issue 1, 1-7

Abstract: Abstract Leguminous plants establish a symbiosis with rhizobia to enable nitrogen fixation in root nodules under the control of the presumed root-to-shoot-to-root negative feedback called autoregulation of nodulation. In Lotus japonicus, autoregulation is mediated by CLE-RS genes that are specifically expressed in the root, and the receptor kinase HAR1 that functions in the shoot. However, the mature functional structures of CLE-RS gene products and the molecular nature of CLE-RS/HAR1 signalling governed by these spatially distant components remain elusive. Here we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain and sequence-dependent manner. In addition, CLE-RS2 glycopeptide specifically produced in the root is found in xylem sap collected from the shoot. We propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of autoregulation of nodulation.

Date: 2013
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DOI: 10.1038/ncomms3191

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