High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics

Benjamin Schmid, Gopi Shah, Nico Scherf, Michael Weber, Konstantin Thierbach, Citlali Pérez Campos, Ingo Roeder, Pia Aanstad and Jan Huisken ()
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Benjamin Schmid: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Gopi Shah: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Nico Scherf: Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74
Michael Weber: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Konstantin Thierbach: Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74
Citlali Pérez Campos: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108
Ingo Roeder: Institute for Medical Informatics and Biometry, Medical School, TU Dresden, Fetscherstr. 74
Pia Aanstad: Institute of Molecular Biology and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Technikerstr. 25
Jan Huisken: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108

Nature Communications, 2013, vol. 4, issue 1, 1-10

Abstract: Abstract The ever-increasing speed and resolution of modern microscopes make the storage and post-processing of images challenging and prevent thorough statistical analyses in developmental biology. Here, instead of deploying massive storage and computing power, we exploit the spherical geometry of zebrafish embryos by computing a radial maximum intensity projection in real time with a 240-fold reduction in data rate. In our four-lens selective plane illumination microscope (SPIM) setup the development of multiple embryos is recorded in parallel and a map of all labelled cells is obtained for each embryo in

Date: 2013
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DOI: 10.1038/ncomms3207

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