Tubular endocytosis drives remodelling of the apical surface during epithelial morphogenesis in Drosophila

Piotr Fabrowski, Aleksandar S. Necakov, Simone Mumbauer, Eva Loeser, Alessandra Reversi, Sebastian Streichan, John A. G. Briggs and Stefano De Renzis ()
Additional contact information
Piotr Fabrowski: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Aleksandar S. Necakov: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Simone Mumbauer: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Eva Loeser: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Alessandra Reversi: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Sebastian Streichan: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
John A. G. Briggs: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1
Stefano De Renzis: European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstrasse 1

Nature Communications, 2013, vol. 4, issue 1, 1-12

Abstract: Abstract During morphogenesis, remodelling of cell shape requires the expansion or contraction of plasma membrane domains. Here we identify a mechanism underlying the restructuring of the apical surface during epithelial morphogenesis in Drosophila. We show that the retraction of villous protrusions and subsequent apical plasma membrane flattening is an endocytosis-driven morphogenetic process. Quantitation of endogenously tagged GFP::Rab5 dynamics reveals a massive increase in apical endocytosis that correlates with changes in apical morphology. This increase is accompanied by the formation of tubular plasma membrane invaginations that serve as platforms for the de novo generation of Rab5-positive endosomes. We identify the Rab5-effector Rabankyrin-5 as a regulator of this pathway and demonstrate that blocking dynamin activity results in the complete inhibition of tubular endocytosis, in the disappearance of Rab5 endosomes, and in the inhibition of surface flattening. These data collectively demonstrate a requirement for endocytosis in morphogenetic remodelling during epithelial development.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms3244 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3244

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3244

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().