In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique

Laila Ritsma, Nienke Vrisekoop and Jacco van Rheenen ()
Additional contact information
Laila Ritsma: Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht
Nienke Vrisekoop: Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht
Jacco van Rheenen: Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht

Nature Communications, 2013, vol. 4, issue 1, 1-8

Abstract: Abstract Intravital microscopy has been used extensively to study dynamic processes in the context of a living animal; however, only a limited number of fluorescent probes and mouse models are available. By contrast, many dyes and antibodies exist for the immuno-labelling of fixed tissue. Here we report a method that combines the advantages of histochemistry and in vivo imaging by correlating cryosection labelling with corresponding intravital microscopy images (CLIM). Using CLIM, we find that the presence of CD3+ T cells correlates with mammary tumour cell migration. When CD4+ and CD8+ T-cell subsets are depleted, reduced tumour cell migration is observed. From these data we conclude that CLIM is a powerful tool to correlate intravital microscopy data with cryosection labelling data.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms3366 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3366

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3366

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().