A versatile cis-acting inverter module for synthetic translational switches

Kei Endo, Karin Hayashi, Tan Inoue () and Hirohide Saito ()
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Kei Endo: International Cooperative Research Project, Japan Science and Technology Agency
Karin Hayashi: International Cooperative Research Project, Japan Science and Technology Agency
Tan Inoue: International Cooperative Research Project, Japan Science and Technology Agency
Hirohide Saito: International Cooperative Research Project, Japan Science and Technology Agency

Nature Communications, 2013, vol. 4, issue 1, 1-9

Abstract: Abstract Artificial genetic switches have been designed and tuned individually in living cells. A method to directly invert an existing OFF switch to an ON switch should be highly convenient to construct complex circuits from well-characterized modules, but developing such a technique has remained a challenge. Here we present a cis-acting RNA module to invert the function of a synthetic translational OFF switch to an ON switch in mammalian cells. This inversion maintains the property of the parental switch in response to a particular input signal. In addition, we demonstrate simultaneous and specific expression control of both the OFF and ON switches. The module fits the criteria of universality and expands the versatility of mRNA-based information processing systems developed for artificially controlling mammalian cellular behaviour.

Date: 2013
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DOI: 10.1038/ncomms3393

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