High-resolution three-dimensional mapping of mRNA export through the nuclear pore

Jiong Ma, Zhen Liu, Nicole Michelotti, Sethuramasundaram Pitchiaya, Ram Veerapaneni, John R. Androsavich, Nils G. Walter and Weidong Yang ()
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Jiong Ma: Temple University
Zhen Liu: Temple University
Nicole Michelotti: Single Molecule Analysis Group, University of Michigan
Sethuramasundaram Pitchiaya: Single Molecule Analysis Group, University of Michigan
Ram Veerapaneni: Temple University
John R. Androsavich: Single Molecule Analysis Group, University of Michigan
Nils G. Walter: Single Molecule Analysis Group, University of Michigan
Weidong Yang: Temple University

Nature Communications, 2013, vol. 4, issue 1, 1-9

Abstract: Abstract The flow of genetic information is regulated by selective nucleocytoplasmic transport of messenger RNA:protein complexes (mRNPs) through the nuclear pore complexes (NPCs) of eukaryotic cells. However, the three-dimensional (3D) pathway taken by mRNPs as they transit through the NPC, and the kinetics and selectivity of transport, remain obscure. Here we employ single-molecule fluorescence microscopy with an unprecedented spatiotemporal accuracy of 8 nm and 2 ms to provide new insights into the mechanism of nuclear mRNP export in live human cells. We find that mRNPs exiting the nucleus are decelerated and selected at the centre of the NPC, and adopt a fast-slow-fast diffusion pattern during their brief, ~12 ms, interaction with the NPC. A 3D reconstruction of the export route indicates that mRNPs primarily interact with the periphery on the nucleoplasmic side and in the centre of the NPC, without entering the central axial conduit utilized for passive diffusion of small molecules, and eventually dissociate on the cytoplasmic side.

Date: 2013
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DOI: 10.1038/ncomms3414

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