Dependence of fertility on kisspeptin–Gpr54 signaling at the GnRH neuron

Milen Kirilov, Jenny Clarkson, Xinhuai Liu, Juan Roa, Pauline Campos, Rob Porteous, Günther Schütz and Allan E. Herbison ()
Additional contact information
Milen Kirilov: Molecular Biology of the Cell I, German Cancer Research Center, Im Neuenheimer Feld 280
Jenny Clarkson: Centre for Neuroendocrinology, University of Otago School of Medical Sciences
Xinhuai Liu: Centre for Neuroendocrinology, University of Otago School of Medical Sciences
Juan Roa: Centre for Neuroendocrinology, University of Otago School of Medical Sciences
Pauline Campos: Centre for Neuroendocrinology, University of Otago School of Medical Sciences
Rob Porteous: Centre for Neuroendocrinology, University of Otago School of Medical Sciences
Günther Schütz: Molecular Biology of the Cell I, German Cancer Research Center, Im Neuenheimer Feld 280
Allan E. Herbison: Centre for Neuroendocrinology, University of Otago School of Medical Sciences

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract Signaling between kisspeptin and its receptor, G-protein-coupled receptor 54 (Gpr54), is now recognized as being essential for normal fertility. However, the key cellular location of kisspeptin–Gpr54 signaling is unknown. Here we create a mouse with a GnRH neuron-specific deletion of Gpr54 to assess the role of gonadotropin-releasing hormone (GnRH) neurons. Mutant mice are infertile, fail to go through puberty and exhibit markedly reduced gonadal size and follicle-stimulating hormone levels alongside GnRH neurons that are unresponsive to kisspeptin. In an attempt to rescue the infertile phenotype of global Gpr54−/− mutants, we use BAC transgenesis to target Gpr54 to the GnRH neurons. This results in mice with normal puberty onset, estrous cyclicity, fecundity and a recovery of kisspeptin’s stimulatory action upon GnRH neurons. Using complimentary cell-specific knockout and knockin approaches we demonstrate here that the GnRH neuron is the key site of kisspeptin–Gpr54 signaling for fertility.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms3492 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3492

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3492

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().