Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio

Mathew Tantama, Juan Ramón Martínez-François, Rebecca Mongeon and Gary Yellen ()
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Mathew Tantama: Harvard Medical School
Juan Ramón Martínez-François: Harvard Medical School
Rebecca Mongeon: Harvard Medical School
Gary Yellen: Harvard Medical School

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract The ATP:ADP ratio is a critical parameter of cellular energy status that regulates many metabolic activities. Here we report an optimized genetically encoded fluorescent biosensor, PercevalHR, that senses the ATP:ADP ratio. PercevalHR is tuned to the range of intracellular ATP:ADP expected in mammalian cells, and it can be used with one- or two-photon microscopy in live samples. We use PercevalHR to visualize activity-dependent changes in ATP:ADP when neurons are exposed to multiple stimuli, demonstrating that it is a sensitive reporter of physiological changes in energy consumption and production. We also use PercevalHR to visualize intracellular ATP:ADP while simultaneously recording currents from ATP-sensitive potassium (KATP) channels in single cells, showing that PercevalHR enables the study of coordinated variation in ATP:ADP and KATP channel open probability in intact cells. With its ability to monitor changes in cellular energetics within seconds, PercevalHR should be a versatile tool for metabolic research.

Date: 2013
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3550

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DOI: 10.1038/ncomms3550

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