Mitochondrial AtPAM16 is required for plant survival and the negative regulation of plant immunity

Yan Huang, Xuejin Chen, Yanan Liu, Charlotte Roth, Charles Copeland, Heather E. McFarlane, Shuai Huang, Volker Lipka, Marcel Wiermer and Xin Li ()
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Yan Huang: Michael Smith Laboratories, University of British Columbia
Xuejin Chen: Michael Smith Laboratories, University of British Columbia
Yanan Liu: University of British Columbia
Charlotte Roth: Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University Göttingen
Charles Copeland: Michael Smith Laboratories, University of British Columbia
Heather E. McFarlane: University of British Columbia
Shuai Huang: Michael Smith Laboratories, University of British Columbia
Volker Lipka: Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University Göttingen
Marcel Wiermer: Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University Göttingen
Xin Li: Michael Smith Laboratories, University of British Columbia

Nature Communications, 2013, vol. 4, issue 1, 1-13

Abstract: Abstract Proteins containing nucleotide-binding and leucine-rich repeat domains (NB-LRRs) serve as immune receptors in plants and animals. Negative regulation of immunity mediated by NB-LRR proteins is crucial, as their overactivation often leads to autoimmunity. Here we describe a new mu tant, s nc1-e nhancing (muse) forward genetic screen, targeting unknown negative regulators of NB-LRR-mediated resistance in Arabidopsis. From the screen, we identify MUSE5, which is renamed as AtPAM16 because it encodes the ortholog of yeast PAM16, part of the mitochondrial inner membrane protein import motor. Consistently, AtPAM16–GFP localizes to the mitochondrial inner membrane. AtPAM16L is a paralog of AtPAM16. Double mutant Atpam16-1 Atpam16l is lethal, indicating that AtPAM16 function is essential. Single mutant Atpam16 plants exhibit a smaller size and enhanced resistance against virulent pathogens. They also display elevated reactive oxygen species (ROS) accumulation. Therefore, AtPAM16 seems to be involved in importing a negative regulator of plant immunity into mitochondria, thus protecting plants from over-accumulation of ROS and preventing autoimmunity.

Date: 2013
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DOI: 10.1038/ncomms3558

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