Real-time in vivo imaging of invasive- and biomaterial-associated bacterial infections using fluorescently labelled vancomycin

Marleen van Oosten, Tina Schäfer, Joost A. C. Gazendam, Knut Ohlsen, Eleni Tsompanidou, Marcus C. de Goffau, Hermie J. M. Harmsen, Lucia M. A. Crane, Ed Lim, Kevin P. Francis, Lael Cheung, Michael Olive, Vasilis Ntziachristos, Jan Maarten van Dijl () and Gooitzen M. van Dam
Additional contact information
Marleen van Oosten: University of Groningen, University Medical Center Groningen (UMCG)
Tina Schäfer: Institut für Molekulare Infektionsbiologie, Universität Würzburg
Joost A. C. Gazendam: University of Groningen, University Medical Center Groningen (UMCG)
Knut Ohlsen: Institut für Molekulare Infektionsbiologie, Universität Würzburg
Eleni Tsompanidou: University of Groningen, University Medical Center Groningen (UMCG)
Marcus C. de Goffau: University of Groningen, University Medical Center Groningen (UMCG)
Hermie J. M. Harmsen: University of Groningen, University Medical Center Groningen (UMCG)
Lucia M. A. Crane: BioOptical Imaging Center, University of Groningen, UMCG
Ed Lim: Caliper—a PerkinElmer Company
Kevin P. Francis: Caliper—a PerkinElmer Company
Lael Cheung: LI-COR Biosciences
Michael Olive: LI-COR Biosciences
Vasilis Ntziachristos: Technische Universität München & Helmholtz Zentrum
Jan Maarten van Dijl: University of Groningen, University Medical Center Groningen (UMCG)
Gooitzen M. van Dam: BioOptical Imaging Center, University of Groningen, UMCG

Nature Communications, 2013, vol. 4, issue 1, 1-8

Abstract: Abstract Invasive and biomaterial-associated infections in humans are often difficult to diagnose and treat. Here, guided by recent advances in clinically relevant optical imaging technologies, we explore the use of fluorescently labelled vancomycin (vanco-800CW) to specifically target and detect infections caused by Gram-positive bacteria. The application potential of vanco-800CW for real-time in vivo imaging of bacterial infections is assessed in a mouse myositis model and a human post-mortem implant model. We show that vanco-800CW can specifically detect Gram-positive bacterial infections in our mouse myositis model, discriminate bacterial infections from sterile inflammation in vivo and detect biomaterial-associated infections in the lower leg of a human cadaver. We conclude that vanco-800CW has a high potential for enhanced non-invasive diagnosis of infections with Gram-positive bacteria and is a promising candidate for early-phase clinical trials.

Date: 2013
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DOI: 10.1038/ncomms3584

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