Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis

Philippe Ranocha, Oana Dima, Réka Nagy, Judith Felten, Claire Corratgé-Faillie, Ondřej Novák, Kris Morreel, Benoît Lacombe, Yves Martinez, Stephanie Pfrunder, Xu Jin, Jean-Pierre Renou, Jean-Baptiste Thibaud, Karin Ljung, Urs Fischer, Enrico Martinoia, Wout Boerjan and Deborah Goffner ()
Additional contact information
Philippe Ranocha: Université de Toulouse; UPS; UMR 5546, Laboratoire de Recherche en Sciences Végétales
Oana Dima: VIB
Réka Nagy: Institut of Plant Biology, University of Zürich
Judith Felten: Umeå Plant Science Center, Swedish University of Agricultural Sciences
Claire Corratgé-Faillie: Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 CNRS/UMR 0386 INRA/SupAgro-M/UM2, Institut Claude Grignon
Ondřej Novák: Umeå Plant Science Center, Swedish University of Agricultural Sciences
Kris Morreel: VIB
Benoît Lacombe: Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 CNRS/UMR 0386 INRA/SupAgro-M/UM2, Institut Claude Grignon
Yves Martinez: Université de Toulouse; UPS; UMR 5546, Laboratoire de Recherche en Sciences Végétales
Stephanie Pfrunder: Institut of Plant Biology, University of Zürich
Xu Jin: Umeå Plant Science Center, Swedish University of Agricultural Sciences
Jean-Pierre Renou: Institut de Recherches en Horticulture et Semences, INRA/ACO/Université d'Angers
Jean-Baptiste Thibaud: Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 CNRS/UMR 0386 INRA/SupAgro-M/UM2, Institut Claude Grignon
Karin Ljung: Umeå Plant Science Center, Swedish University of Agricultural Sciences
Urs Fischer: Umeå Plant Science Center, Swedish University of Agricultural Sciences
Enrico Martinoia: Institut of Plant Biology, University of Zürich
Wout Boerjan: VIB
Deborah Goffner: Université de Toulouse; UPS; UMR 5546, Laboratoire de Recherche en Sciences Végétales

Nature Communications, 2013, vol. 4, issue 1, 1-9

Abstract: Abstract The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation.

Date: 2013
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DOI: 10.1038/ncomms3625

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