Localized cell stimulation by nitric oxide using a photoactive porous coordination polymer platform

Stéphane Diring, Dan Ohtan Wang, Chiwon Kim, Mio Kondo, Yong Chen, Susumu Kitagawa, Ken-ichiro Kamei () and Shuhei Furukawa ()
Additional contact information
Stéphane Diring: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Dan Ohtan Wang: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Chiwon Kim: Graduate School of Engineering, Kyoto University
Mio Kondo: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Yong Chen: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Susumu Kitagawa: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Ken-ichiro Kamei: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University
Shuhei Furukawa: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University

Nature Communications, 2013, vol. 4, issue 1, 1-8

Abstract: Abstract Functional cellular substrates for localized cell stimulation by small molecules provide an opportunity to control and monitor cell signalling networks chemically in time and space. However, despite improvements in the controlled delivery of bioactive compounds, the precise localization of gaseous biomolecules at the single-cell level remains challenging. Here we target nitric oxide, a crucial signalling molecule with site-specific and concentration-dependent activities, and we report a synthetic strategy for developing spatiotemporally controllable nitric oxide-releasing platforms based on photoactive porous coordination polymers. By organizing molecules with poor reactivity into polymer structures, we observe increased photoreactivity and adjustable release using light irradiation. We embed photoactive polymer crystals in a biocompatible matrix and achieve precisely controlled nitric oxide delivery at the cellular level via localized two-photon laser activation. The biological relevance of the exogenous nitric oxide produced by this strategy is evidenced by an intracellular change in calcium concentration, mediated by nitric oxide-responsive plasma membrane channel proteins.

Date: 2013
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/ncomms3684 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3684

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3684

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().