Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes

Qing Wei, Qingwen Xu, Yuxia Zhang, Yujie Li, Qing Zhang, Zeng Hu, Peter C. Harris, Vicente E. Torres, Kun Ling () and Jinghua Hu ()
Additional contact information
Qing Wei: Mayo Clinic
Qingwen Xu: Mayo Clinic
Yuxia Zhang: Mayo Clinic
Yujie Li: Mayo Clinic
Qing Zhang: Mayo Clinic
Zeng Hu: Mayo Clinic
Peter C. Harris: Mayo Clinic
Vicente E. Torres: Mayo Clinic
Kun Ling: Mayo Clinic
Jinghua Hu: Mayo Clinic

Nature Communications, 2013, vol. 4, issue 1, 1-10

Abstract: Abstract Sensory organelle cilia have critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet, how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the ciliary base. We cloned dyf-19, the Caenorhabditis elegans homologue of human FBF1, in a whole-genome screen for ciliogenesis mutants. DYF-19 localizes specifically to transition fibres and interacts directly with the IFT-B component DYF-11/IFT54. Although not a structural component of transition fibres, DYF-19 is required for the transit of assembled IFT particles through the ciliary base. Furthermore, we found that human FBF1 shares conserved localization and function with its worm counterpart. We conclude that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery.

Date: 2013
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DOI: 10.1038/ncomms3750

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