miR-1 and miR-206 target different genes to have opposing roles during angiogenesis in zebrafish embryos

Cheng-Yung Lin, Hung-Chieh Lee, Chuan-Yang Fu, Yu-Yun Ding, Jie-Shin Chen, Ming-Hsuan Lee, Wei-Jhen Huang and Huai-Jen Tsai ()
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Cheng-Yung Lin: Institute of Molecular and Cellular Biology, National Taiwan University
Hung-Chieh Lee: Institute of Molecular and Cellular Biology, National Taiwan University
Chuan-Yang Fu: Institute of Molecular and Cellular Biology, National Taiwan University
Yu-Yun Ding: Institute of Molecular and Cellular Biology, National Taiwan University
Jie-Shin Chen: Institute of Molecular and Cellular Biology, National Taiwan University
Ming-Hsuan Lee: Institute of Molecular and Cellular Biology, National Taiwan University
Wei-Jhen Huang: Institute of Molecular and Cellular Biology, National Taiwan University
Huai-Jen Tsai: Institute of Molecular and Cellular Biology, National Taiwan University

Nature Communications, 2013, vol. 4, issue 1, 1-11

Abstract: Abstract As miR-1 and miR-206 share identical seed sequences, they are commonly speculated to target the same gene. Here, we identify an mRNA encoding seryl-tRNA synthetase (SARS), which is targeted by miR-1, but refractory to miR-206. SARS is increased in miR-1-knockdown embryos, but it remains unchanged in the miR-206 knockdown. Either miR-1 knockdown or sars overexpression results in a failure to develop some blood vessels and a decrease in vascular endothelial growth factor Aa (VegfAa) expression. In contrast, sars knockdown leads to an increase of VegfAa expression and abnormal branching of vessels, similar to the phenotypes of vegfaa-overexpressed embryos, suggesting that miR-1 induces angiogenesis by repressing SARS. Unlike the few endothelial cells observed in the miR-1-knockdown embryos, knockdown of miR-206 leads to abnormal branching of vessels accompanied by an increase in endothelial cells and VegfAa. Therefore, we propose that miR-1 and miR-206 target different genes and thus have opposing roles during embryonic angiogenesis in zebrafish.

Date: 2013
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DOI: 10.1038/ncomms3829

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