A quantitative telomeric chromatin isolation protocol identifies different telomeric states

Larissa Grolimund, Eric Aeby, Romain Hamelin, Florence Armand, Diego Chiappe, Marc Moniatte and Joachim Lingner ()
Additional contact information
Larissa Grolimund: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Eric Aeby: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Romain Hamelin: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Florence Armand: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Diego Chiappe: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Marc Moniatte: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences
Joachim Lingner: EPFL-Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences

Nature Communications, 2013, vol. 4, issue 1, 1-12

Abstract: Abstract Telomere composition changes during tumourigenesis, aging and in telomere syndromes in a poorly defined manner. Here we develop a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified and analysed by mass spectrometry. QTIP involves stable isotope labelling by amino acids in cell culture (SILAC) to compare and identify quantitative differences in telomere protein composition of cells from various states. With QTIP, we specifically enrich telomeric DNA and all shelterin components. We validate the method characterizing changes at dysfunctional telomeres, and identify and validate known, as well as novel telomere-associated polypeptides including all THO subunits, SMCHD1 and LRIF1. We apply QTIP to long and short telomeres and detect increased density of SMCHD1 and LRIF1 and increased association of the shelterins TRF1, TIN2, TPP1 and POT1 with long telomeres. Our results validate QTIP to study telomeric states during normal development and in disease.

Date: 2013
References: Add references at CitEc
Citations: View citations in EconPapers (3)

Downloads: (external link)
https://www.nature.com/articles/ncomms3848 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3848

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3848

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().