An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

Ken-ichi Nomura, Subash C.B. Gopinath, Thangavel Lakshmipriya, Nobuko Fukuda, Xiaomin Wang and Makoto Fujimaki ()
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Ken-ichi Nomura: Flexible Electronics Research Center, National Institute of Advanced Industrial Science and Technology (AIST)
Subash C.B. Gopinath: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Thangavel Lakshmipriya: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Nobuko Fukuda: Flexible Electronics Research Center, National Institute of Advanced Industrial Science and Technology (AIST)
Xiaomin Wang: Nano-electronics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
Makoto Fujimaki: Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

Nature Communications, 2013, vol. 4, issue 1, 1-7

Abstract: Abstract Surface plasmon excitation provides stronger enhancement of the fluorescence intensity and better sensitivity than other sensing approaches but requires optimal positioning of a prism to ensure optimum output of the incident light. Here we describe a simple, highly sensitive optical sensing system combining surface plasmon excitation and fluorescence to address this limitation. V-shaped fluidic channels are employed to mimic the functions of a prism, sensing plate, and flow channel in a single setup. Superior performance is demonstrated for different biomolecular recognition reactions on a self-assembled monolayer, and the sensitivity reaches 100 fM for biotin–streptavidin interactions. Using an antibody as a probe, we demonstrate the detection of intact influenza viruses at 0.2 HA units ml−1 levels. The convenient sensing system developed here has the advantages of being prism-free and requiring less sample (1–2 μl), making this platform suitable for use in situations requiring low sample volumes.

Date: 2013
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DOI: 10.1038/ncomms3855

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