miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Xiaoping Su, Cheng Qian, Qian Zhang, Jin Hou, Yan Gu, Yanmei Han, Yongjian Chen, Minghong Jiang and Xuetao Cao ()
Additional contact information
Xiaoping Su: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Cheng Qian: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Qian Zhang: Chinese Academy of Medical Sciences
Jin Hou: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Yan Gu: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Yanmei Han: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Yongjian Chen: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University
Minghong Jiang: Chinese Academy of Medical Sciences
Xuetao Cao: National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University

Nature Communications, 2013, vol. 4, issue 1, 1-12

Abstract: Abstract Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSCs and DCs at different differentiation stages. miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, suggesting that miR-30b is a negative regulator of immune response. We also identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and systematically compare them with DCs cultured in vitro. These results provide a resource for studying roles of miRNAs in stem cell biology, development and functional regulation of DC subsets.

Date: 2013
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms3903 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3903

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3903

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().