Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs

Carolina Villarroya-Beltri, Cristina Gutiérrez-Vázquez, Fátima Sánchez-Cabo, Daniel Pérez-Hernández, Jesús Vázquez, Noa Martin-Cofreces, Dannys Jorge Martinez-Herrera, Alberto Pascual-Montano, María Mittelbrunn and Francisco Sánchez-Madrid ()
Additional contact information
Carolina Villarroya-Beltri: Centro Nacional de Investigaciones Cardiovasculares
Cristina Gutiérrez-Vázquez: Centro Nacional de Investigaciones Cardiovasculares
Fátima Sánchez-Cabo: Centro Nacional de Investigaciones Cardiovasculares
Daniel Pérez-Hernández: Centro Nacional de Investigaciones Cardiovasculares
Jesús Vázquez: Centro Nacional de Investigaciones Cardiovasculares
Noa Martin-Cofreces: Servicio de Inmunología, Hospital de la Princesa
Dannys Jorge Martinez-Herrera: National Center for Biotechnology-CSIC
Alberto Pascual-Montano: National Center for Biotechnology-CSIC
María Mittelbrunn: Centro Nacional de Investigaciones Cardiovasculares
Francisco Sánchez-Madrid: Centro Nacional de Investigaciones Cardiovasculares

Nature Communications, 2013, vol. 4, issue 1, 1-10

Abstract: Abstract Exosomes are released by most cells to the extracellular environment and are involved in cell-to-cell communication. Exosomes contain specific repertoires of mRNAs, microRNAs (miRNAs) and other non-coding RNAs that can be functionally transferred to recipient cells. However, the mechanisms that control the specific loading of RNA species into exosomes remain unknown. Here we describe sequence motifs present in miRNAs that control their localization into exosomes. The protein heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) specifically binds exosomal miRNAs through the recognition of these motifs and controls their loading into exosomes. Moreover, hnRNPA2B1 in exosomes is sumoylated, and sumoylation controls the binding of hnRNPA2B1 to miRNAs. The loading of miRNAs into exosomes can be modulated by mutagenesis of the identified motifs or changes in hnRNPA2B1 expression levels. These findings identify hnRNPA2B1 as a key player in miRNA sorting into exosomes and provide potential tools for the packaging of selected regulatory RNAs into exosomes and their use in biomedical applications.

Date: 2013
References: Add references at CitEc
Citations: View citations in EconPapers (4)

Downloads: (external link)
https://www.nature.com/articles/ncomms3980 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:4:y:2013:i:1:d:10.1038_ncomms3980

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms3980

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().