Light-regulated gene repositioning in Arabidopsis

Chun-Miao Feng, Yongjian Qiu, Elise K. Van Buskirk, Emily J. Yang and Meng Chen ()
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Chun-Miao Feng: Duke University
Yongjian Qiu: Duke University
Elise K. Van Buskirk: Duke University
Emily J. Yang: Duke University
Meng Chen: Duke University

Nature Communications, 2014, vol. 5, issue 1, 1-9

Abstract: Abstract Plant genomes are extremely sensitive to, and can be developmentally reprogrammed by environmental light cues. Here using rolling-circle amplification of gene-specific circularizable oligonucleotides coupled with fluorescence in situ hybridization, we demonstrate that light triggers a rapid repositioning of the Arabidopsis light-inducible chlorophyll a/b-binding proteins (CAB) locus from the nuclear interior to the nuclear periphery during its transcriptional activation. CAB repositioning is mediated by the red/far-red photoreceptors phytochromes (PHYs) and is inhibited by repressors of PHY signalling, including COP1, DET1 and PIFs. CAB repositioning appears to be a separate regulatory step occurring before its full transcriptional activation. Moreover, the light-inducible loci RBCS, PC and GUN5 undergo similar repositioning behaviour during their transcriptional activation. Our study supports a light-dependent gene regulatory mechanism in which PHYs activate light-inducible loci by relocating them to the nuclear periphery; it also provides evidence for the biological importance of gene positioning in the plant kingdom.

Date: 2014
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DOI: 10.1038/ncomms4027

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