Imaging live cell in micro-liquid enclosure by X-ray laser diffraction

Takashi Kimura, Yasumasa Joti, Akemi Shibuya, Changyong Song, Sangsoo Kim, Kensuke Tono, Makina Yabashi, Masatada Tamakoshi, Toshiyuki Moriya, Tairo Oshima, Tetsuya Ishikawa, Yoshitaka Bessho () and Yoshinori Nishino ()
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Takashi Kimura: Research Institute for Electronic Science, Hokkaido University
Yasumasa Joti: Japan Synchrotron Radiation Research Institute/SPring-8
Akemi Shibuya: RIKEN SPring-8 Center
Changyong Song: RIKEN SPring-8 Center
Sangsoo Kim: RIKEN SPring-8 Center
Kensuke Tono: Japan Synchrotron Radiation Research Institute/SPring-8
Makina Yabashi: RIKEN SPring-8 Center
Masatada Tamakoshi: Tokyo University of Pharmacy and Life Sciences
Toshiyuki Moriya: Institute of Environmental Microbiology, Kyowa-kako Co. Ltd.
Tairo Oshima: Institute of Environmental Microbiology, Kyowa-kako Co. Ltd.
Tetsuya Ishikawa: RIKEN SPring-8 Center
Yoshitaka Bessho: RIKEN SPring-8 Center
Yoshinori Nishino: Research Institute for Electronic Science, Hokkaido University

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation damage processes. In bioimaging, it is essential to keep the sample close to its natural state. Conventional high-resolution imaging, however, suffers from severe radiation damage that hinders live cell imaging. Here we present a method for capturing snapshots of live cells kept in a micro-liquid enclosure array by X-ray laser diffraction. We place living Microbacterium lacticum cells in an enclosure array and successively expose each enclosure to a single X-ray laser pulse from the SPring-8 Angstrom Compact Free-Electron Laser. The enclosure itself works as a guard slit and allows us to record a coherent diffraction pattern from a weakly-scattering submicrometre-sized cell with a clear fringe extending up to a 28-nm full-period resolution. The reconstructed image reveals living whole-cell structures without any staining, which helps advance understanding of intracellular phenomena.

Date: 2014
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DOI: 10.1038/ncomms4052

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