Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells

Ruifeng Yang, Ying Zheng, Michelle Burrows, Shujing Liu, Zhi Wei, Arben Nace, Wei Guo, Suresh Kumar, George Cotsarelis and Xiaowei Xu ()
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Ruifeng Yang: Perelman School of Medicine, University of Pennsylvania
Ying Zheng: Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania
Michelle Burrows: Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania
Shujing Liu: Perelman School of Medicine, University of Pennsylvania
Zhi Wei: New Jersey Institute of Technology
Arben Nace: Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania
Wei Guo: University of Pennsylvania
Suresh Kumar: Perelman School of Medicine, University of Pennsylvania
George Cotsarelis: Kligman Laboratories, Perelman School of Medicine, University of Pennsylvania
Xiaowei Xu: Perelman School of Medicine, University of Pennsylvania

Nature Communications, 2014, vol. 5, issue 1, 1-11

Abstract: Abstract Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. The isolation and propagation of human EpSCs for tissue engineering purposes remains a challenge. Here we develop a strategy to differentiate human iPSCs (hiPSCs) into CD200+/ITGA6+ EpSCs that can reconstitute the epithelial components of the hair follicle and interfollicular epidermis. The hiPSC-derived CD200+/ITGA6+ cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. Human iPSC-derived CD200+/ITGA6+ cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. The regenerated hair follicles possess a KRT15+ stem cell population and produce hair shafts expressing hair-specific keratins. These results suggest an approach for generating large numbers of human EpSCs for tissue engineering and new treatments for hair loss, wound healing and other degenerative skin disorders.

Date: 2014
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DOI: 10.1038/ncomms4071

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