Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production

John Blazeck, Andrew Hill, Leqian Liu, Rebecca Knight, Jarrett Miller, Anny Pan, Peter Otoupal and Hal S. Alper ()
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John Blazeck: The University of Texas at Austin
Andrew Hill: The University of Texas at Austin
Leqian Liu: The University of Texas at Austin
Rebecca Knight: Section of Molecular, Cell and Developmental Biology, The University of Texas at Austin
Jarrett Miller: The University of Texas at Austin
Anny Pan: The University of Texas at Austin
Peter Otoupal: The University of Texas at Austin
Hal S. Alper: The University of Texas at Austin

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract Economic feasibility of biosynthetic fuel and chemical production hinges upon harnessing metabolism to achieve high titre and yield. Here we report a thorough genotypic and phenotypic optimization of an oleaginous organism to create a strain with significant lipogenesis capability. Specifically, we rewire Yarrowia lipolytica’s native metabolism for superior de novo lipogenesis by coupling combinatorial multiplexing of lipogenesis targets with phenotypic induction. We further complete direct conversion of lipid content into biodiesel. Tri-level metabolic control results in saturated cells containing upwards of 90% lipid content and titres exceeding 25 g l−1 lipids, which represents a 60-fold improvement over parental strain and conditions. Through this rewiring effort, we advance fundamental understanding of lipogenesis, demonstrate non-canonical environmental and intracellular stimuli and uncouple lipogenesis from nitrogen starvation. The high titres and carbon-source independent nature of this lipogenesis in Y. lipolytica highlight the potential of this organism as a platform for efficient oleochemical production.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4131

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DOI: 10.1038/ncomms4131

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