Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms

Daniel L. Bellin, Hassan Sakhtah, Jacob K. Rosenstein, Peter M. Levine, Jordan Thimot, Kevin Emmett, Lars E. P. Dietrich () and Kenneth L. Shepard ()
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Daniel L. Bellin: Columbia University
Hassan Sakhtah: Columbia University
Jacob K. Rosenstein: School of Engineering, Brown University
Peter M. Levine: University of Waterloo
Jordan Thimot: Columbia University
Kevin Emmett: Columbia University
Lars E. P. Dietrich: Columbia University
Kenneth L. Shepard: Columbia University

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites that are produced by microbial biofilms and can affect their development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. ‘Images’ over a 3.25 × 0.9 mm2 area can be captured with a diffusion-limited spatial resolution of 750 μm. We demonstrate that square wave voltammetry can be used to detect, identify and quantify (for concentrations as low as 2.6 μM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4256

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DOI: 10.1038/ncomms4256

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