Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Uwe Weierstall (), Daniel James, Chong Wang, Thomas A. White, Dingjie Wang, Wei Liu, John C. H. Spence, R. Bruce Doak, Garrett Nelson, Petra Fromme, Raimund Fromme, Ingo Grotjohann, Christopher Kupitz, Nadia A. Zatsepin, Haiguang Liu, Shibom Basu, Daniel Wacker, Gye Won Han, Vsevolod Katritch, Sébastien Boutet, Marc Messerschmidt, Garth J. Williams, Jason E. Koglin, M. Marvin Seibert, Markus Klinker, Cornelius Gati, Robert L. Shoeman, Anton Barty, Henry N. Chapman, Richard A. Kirian, Kenneth R. Beyerlein, Raymond C. Stevens, Dianfan Li, Syed T. A. Shah, Nicole Howe, Martin Caffrey and Vadim Cherezov ()
Additional contact information
Uwe Weierstall: Arizona State University
Daniel James: Arizona State University
Chong Wang: The Scripps Research Institute
Thomas A. White: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Dingjie Wang: Arizona State University
Wei Liu: The Scripps Research Institute
John C. H. Spence: Arizona State University
R. Bruce Doak: Arizona State University
Garrett Nelson: Arizona State University
Petra Fromme: Arizona State University
Raimund Fromme: Arizona State University
Ingo Grotjohann: Arizona State University
Christopher Kupitz: Arizona State University
Nadia A. Zatsepin: Arizona State University
Haiguang Liu: Arizona State University
Shibom Basu: Arizona State University
Daniel Wacker: The Scripps Research Institute
Gye Won Han: The Scripps Research Institute
Vsevolod Katritch: The Scripps Research Institute
Sébastien Boutet: SLAC National Accelerator Laboratory, 2575 Sand Hill Road
Marc Messerschmidt: SLAC National Accelerator Laboratory, 2575 Sand Hill Road
Garth J. Williams: SLAC National Accelerator Laboratory, 2575 Sand Hill Road
Jason E. Koglin: SLAC National Accelerator Laboratory, 2575 Sand Hill Road
M. Marvin Seibert: SLAC National Accelerator Laboratory, 2575 Sand Hill Road
Markus Klinker: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Cornelius Gati: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Robert L. Shoeman: Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29
Anton Barty: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Henry N. Chapman: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Richard A. Kirian: Arizona State University
Kenneth R. Beyerlein: Center for Free-Electron Laser Science, DESY, Notkestrasse 85
Raymond C. Stevens: The Scripps Research Institute
Dianfan Li: School of Medicine and School of Biochemistry and Immunology, Trinity College
Syed T. A. Shah: School of Medicine and School of Biochemistry and Immunology, Trinity College
Nicole Howe: School of Medicine and School of Biochemistry and Immunology, Trinity College
Martin Caffrey: School of Medicine and School of Biochemistry and Immunology, Trinity College
Vadim Cherezov: The Scripps Research Institute

Nature Communications, 2014, vol. 5, issue 1, 1-6

Abstract: Abstract Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Date: 2014
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DOI: 10.1038/ncomms4309

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