A TAL effector repeat architecture for frameshift binding

Annekatrin Richter, Jana Streubel, Christina Blücher, Boris Szurek, Maik Reschke, Jan Grau and Jens Boch ()
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Annekatrin Richter: Martin Luther University Halle-Wittenberg
Jana Streubel: Martin Luther University Halle-Wittenberg
Christina Blücher: Martin Luther University Halle-Wittenberg
Boris Szurek: UMR 186 IRD-UM2-Cirad ‘Résistance des Plantes aux Bioagresseurs’
Maik Reschke: Martin Luther University Halle-Wittenberg
Jan Grau: Institute of Computer Science, Martin Luther University Halle-Wittenberg, von-Seckendorff-Platz 1
Jens Boch: Martin Luther University Halle-Wittenberg

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract Transcription activator-like effectors (TALEs) are important Xanthomonas virulence factors that bind DNA via a unique tandem 34-amino-acid repeat domain to induce expression of plant genes. So far, TALE repeats are described to bind as a consecutive array to a consecutive DNA sequence, in which each repeat independently recognizes a single DNA base. This modular protein architecture enables the design of any desired DNA-binding specificity for biotechnology applications. Here we report that natural TALE repeats of unusual amino-acid sequence length break the strict one repeat-to-one base pair binding mode and introduce a local flexibility to TALE–DNA binding. This flexibility allows TALEs and TALE nucleases to recognize target sequence variants with single nucleotide deletions. The flexibility also allows TALEs to activate transcription at allelic promoters that otherwise confer resistance to the host plant.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4447

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DOI: 10.1038/ncomms4447

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