High-efficiency motor neuron differentiation from human pluripotent stem cells and the function of Islet-1

Qiuhao Qu, Dong Li, Kathleen R. Louis, Xiangzhen Li, Hong Yang, Qinyu Sun, Shane R. Crandall, Stephanie Tsang, Jiaxi Zhou, Charles L. Cox, Jianjun Cheng and Fei Wang ()
Additional contact information
Qiuhao Qu: Department of Cell and Developmental Biology
Dong Li: Department of Cell and Developmental Biology
Kathleen R. Louis: University of Illinois at Urbana-Champaign
Xiangzhen Li: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences
Hong Yang: Department of Cell and Developmental Biology
Qinyu Sun: Department of Cell and Developmental Biology
Shane R. Crandall: University of Illinois at Urbana-Champaign
Stephanie Tsang: Department of Cell and Developmental Biology
Jiaxi Zhou: State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
Charles L. Cox: University of Illinois at Urbana-Champaign
Jianjun Cheng: University of Illinois at Urbana-Champaign
Fei Wang: Department of Cell and Developmental Biology

Nature Communications, 2014, vol. 5, issue 1, 1-13

Abstract: Abstract Efficient derivation of large-scale motor neurons (MNs) from human pluripotent stem cells is central to the understanding of MN development, modelling of MN disorders in vitro and development of cell-replacement therapies. Here we develop a method for rapid (20 days) and highly efficient (~70%) differentiation of mature and functional MNs from human pluripotent stem cells by tightly modulating neural patterning temporally at a previously undefined primitive neural progenitor stage. This method also allows high-yield (>250%) MN production in chemically defined adherent cultures. Furthermore, we show that Islet-1 is essential for formation of mature and functional human MNs, but, unlike its mouse counterpart, does not regulate cell survival or suppress the V2a interneuron fate. Together, our discoveries improve the strategy for MN derivation, advance our understanding of human neural specification and MN development, and provide invaluable tools for human developmental studies, drug discovery and regenerative medicine.

Date: 2014
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms4449 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4449

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms4449

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().