One-step pipetting and assembly of encoded chemical-laden microparticles for high-throughput multiplexed bioassays

Su Eun Chung, Jiyun Kim, Dong Yoon Oh, Younghoon Song, Sung Hoon Lee, Seungki Min and Sunghoon Kwon ()
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Su Eun Chung: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
Jiyun Kim: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
Dong Yoon Oh: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
Younghoon Song: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University
Sung Hoon Lee: Seoul National University
Seungki Min: Seoul National University
Sunghoon Kwon: Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University

Nature Communications, 2014, vol. 5, issue 1, 1-12

Abstract: Abstract One quantitative liquid handling method in conventional assay processes is pipetting, which delivers a precise volume of one sample at a time. As this process becomes laborious and time-consuming as the number of samples increases, researchers in individual laboratories need a way to conduct large-scale assays in a reasonable amount of time and at an affordable cost. Here we report a novel handling technique of chemical substances termed ‘partipetting’, which allows the one-step pipetting of various chemical-laden hydrogels. We pipette and assemble various types of encoded chemical-laden microparticles in microwell arrays in parallel. The combination of this heterogeneous particle chip and a cell chip induces the release of the chemicals from the hydrogels and, eventually, the chemicals treat the targets. Based on bioassay applications using partipetting, we show its capability in large-scale bioassays, without the need for high-throughput bioassay resources, owing to a reduction in the assay costs and time.

Date: 2014
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DOI: 10.1038/ncomms4468

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