Bifurcation of the endocytic pathway into Rab5-dependent and -independent transport to the vacuole

Junko Y. Toshima, Show Nishinoaki, Yoshifumi Sato, Wataru Yamamoto, Daiki Furukawa, Daria Elisabeth Siekhaus, Akira Sawaguchi and Jiro Toshima ()
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Junko Y. Toshima: Faculty of Science and Engineering, Waseda University
Show Nishinoaki: Tokyo University of Science
Yoshifumi Sato: Tokyo University of Science
Wataru Yamamoto: Tokyo University of Science
Daiki Furukawa: Tokyo University of Science
Daria Elisabeth Siekhaus: Institute of Science and Technology Austria, Am Campus 1
Akira Sawaguchi: University of Miyazaki Faculty of Medicine
Jiro Toshima: Research Center for RNA Science, RIST, Tokyo University of Science

Nature Communications, 2014, vol. 5, issue 1, 1-11

Abstract: Abstract The yeast Rab5 homologue, Vps21p, is known to be involved both in the vacuolar protein sorting (VPS) pathway from the trans-Golgi network to the vacuole, and in the endocytic pathway from the plasma membrane to the vacuole. However, the intracellular location at which these two pathways converge remains unclear. In addition, the endocytic pathway is not completely blocked in yeast cells lacking all Rab5 genes, suggesting the existence of an unidentified route that bypasses the Rab5-dependent endocytic pathway. Here we show that convergence of the endocytic and VPS pathways occurs upstream of the requirement for Vps21p in these pathways. We also identify a previously unidentified endocytic pathway mediated by the AP-3 complex. Importantly, the AP-3-mediated pathway appears mostly intact in Rab5-disrupted cells, and thus works as an alternative route to the vacuole/lysosome. We propose that the endocytic traffic branches into two routes to reach the vacuole: a Rab5-dependent VPS pathway and a Rab5-independent AP-3-mediated pathway.

Date: 2014
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DOI: 10.1038/ncomms4498

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