Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats

Sebastian Sjöqvist, Philipp Jungebluth, Mei Ling Lim, Johannes C. Haag, Ylva Gustafsson, Greg Lemon, Silvia Baiguera, Miguel Angel Burguillos, Costantino Del Gaudio, Antonio Beltrán Rodríguez, Alexander Sotnichenko, Karolina Kublickiene, Henrik Ullman, Heike Kielstein, Peter Damberg, Alessandra Bianco, Rainer Heuchel, Ying Zhao, Domenico Ribatti, Cristián Ibarra, Bertrand Joseph, Doris A. Taylor and Paolo Macchiarini ()
Additional contact information
Sebastian Sjöqvist: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Philipp Jungebluth: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Mei Ling Lim: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Johannes C. Haag: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Ylva Gustafsson: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Greg Lemon: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Silvia Baiguera: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Miguel Angel Burguillos: Cancer Centrum Karolinska, Karolinska Institutet
Costantino Del Gaudio: Intrauniversitary Consortium for Material Science and Technology (INSTM) Research Unit Tor Vergata, University of Rome
Antonio Beltrán Rodríguez: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86
Alexander Sotnichenko: International Scientific-Research Clinical and Educational Center of Regenerative Medicine, Kuban State Medical University
Karolina Kublickiene: Center for Gender Medicine, Karolinska Institutet
Henrik Ullman: Karolinska Institutet
Heike Kielstein: Faculty of Medicine, Martin Luther University Halle-Wittenberg
Peter Damberg: Intervention and Technology (CLINTEC) Karolinska Institutet
Alessandra Bianco: Intrauniversitary Consortium for Material Science and Technology (INSTM) Research Unit Tor Vergata, University of Rome
Rainer Heuchel: Intervention and Technology (CLINTEC), Karolinska Institutet
Ying Zhao: Intervention and Technology (CLINTEC), Karolinska Institutet
Domenico Ribatti: Neurosciences and Sensory Organs, University of Bari Medical School, National Cancer Institute ‘Giovanni Paolo II’
Cristián Ibarra: Karolinska Institutet
Bertrand Joseph: Cancer Centrum Karolinska, Karolinska Institutet
Doris A. Taylor: Texas Heart Institute, Center for Regenerative Medicine
Paolo Macchiarini: Advanced Center for Translational Regenerative Medicine (ACTREM), Intervention and Technology (CLINTEC), Karolinska Institutet, SE-141 86

Nature Communications, 2014, vol. 5, issue 1, 1-15

Abstract: Abstract A tissue-engineered oesophageal scaffold could be very useful for the treatment of pediatric and adult patients with benign or malignant diseases such as carcinomas, trauma or congenital malformations. Here we decellularize rat oesophagi inside a perfusion bioreactor to create biocompatible biological rat scaffolds that mimic native architecture, resist mechanical stress and induce angiogenesis. Seeded allogeneic mesenchymal stromal cells spontaneously differentiate (proven by gene-, protein and functional evaluations) into epithelial- and muscle-like cells. The reseeded scaffolds are used to orthotopically replace the entire cervical oesophagus in immunocompetent rats. All animals survive the 14-day study period, with patent and functional grafts, and gain significantly more weight than sham-operated animals. Explanted grafts show regeneration of all the major cell and tissue components of the oesophagus including functional epithelium, muscle fibres, nerves and vasculature. We consider the presented tissue-engineered oesophageal scaffolds a significant step towards the clinical application of bioengineered oesophagi.

Date: 2014
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/ncomms4562 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4562

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/ncomms4562

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().