Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Yusuke Yoshioka, Nobuyoshi Kosaka, Yuki Konishi, Hideki Ohta, Hiroyuki Okamoto, Hikaru Sonoda, Ryoji Nonaka, Hirofumi Yamamoto, Hideshi Ishii, Masaki Mori, Koh Furuta, Takeshi Nakajima, Hiroshi Hayashi, Hajime Sugisaki, Hiroko Higashimoto, Takashi Kato, Fumitaka Takeshita and Takahiro Ochiya ()
Additional contact information
Yusuke Yoshioka: National Cancer Center Research Institute
Nobuyoshi Kosaka: National Cancer Center Research Institute
Yuki Konishi: National Cancer Center Research Institute
Hideki Ohta: Shionogi & Co., LTD.
Hiroyuki Okamoto: Shionogi & Co., LTD.
Hikaru Sonoda: Shionogi & Co., LTD.
Ryoji Nonaka: Graduated School of Medicine, Osaka University
Hirofumi Yamamoto: Graduated School of Medicine, Osaka University
Hideshi Ishii: Osaka University, Graduate School of Medicine
Masaki Mori: Graduated School of Medicine, Osaka University
Koh Furuta: National Cancer Center Hospital
Takeshi Nakajima: National Cancer Center Hospital
Hiroshi Hayashi: SRL Inc.
Hajime Sugisaki: SRL Inc.
Hiroko Higashimoto: SRL Inc.
Takashi Kato: Integrative Bioscience and Biomedical Engineering, Graduate School of Advanced Science and Engineering, Waseda University
Fumitaka Takeshita: National Cancer Center Research Institute
Takahiro Ochiya: National Cancer Center Research Institute

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

Date: 2014
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DOI: 10.1038/ncomms4591

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