The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin

Teppei Kitagawa, Kojiro Ishii, Kojiro Takeda and Tomohiro Matsumoto ()
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Teppei Kitagawa: Graduate School of Biostudies, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan
Kojiro Ishii: Graduate School of Frontier Biosciences, Osaka University
Kojiro Takeda: Institute for Integrative Neurobiology, Graduate School of Natural Science, Konan University, Kobe
Tomohiro Matsumoto: Graduate School of Biostudies, Kyoto University, Yoshida-Konoe cho, Sakyo ku, Kyoto 606-8501, Japan

Nature Communications, 2014, vol. 5, issue 1, 1-10

Abstract: Abstract CENP-A, a variant of histone H3, is incorporated into centromeric chromatin and plays a role during kinetochore establishment. In fission yeast, the localization of CENP-A is limited to a region spanning 10–20 kb of the core domain of the centromere. Here, we report a mutant (rpt3-1) in which this region is expanded to 40–70 kb. Likely due to abnormal distribution of CENP-A, this mutant exhibits chromosome instability and enhanced gene silencing. Interestingly, the rpt3+ gene encodes a subunit of the 19S proteasome, which localizes to the nuclear membrane. Although Rpt3 associates with centromeric chromatin, the mutant protein has lost this localization. A loss of the cut8+ gene encoding an anchor of the proteasome to the nuclear membrane causes similar phenotypes as observed in the rpt3-1 mutant. Thus, we propose that the proteasome (or its subcomplex) associates with centromeric chromatin and regulates distribution of CENP-A.

Date: 2014
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DOI: 10.1038/ncomms4597

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