Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER

Kazuo Kurokawa (), Michiyo Okamoto and Akihiko Nakano
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Kazuo Kurokawa: Live Cell Molecular Imaging Research Team, RIKEN Center for Advanced Photonics
Michiyo Okamoto: Live Cell Molecular Imaging Research Team, RIKEN Center for Advanced Photonics
Akihiko Nakano: Live Cell Molecular Imaging Research Team, RIKEN Center for Advanced Photonics

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract Protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus is mediated by coat complex II (COPII) vesicles. It has been believed that COPII vesicles containing cargo are released from the ER exit sites (ERES) into the cytosol and then reach and fuse with the first post-ER compartment, cis-Golgi or ER-to-Golgi intermediate compartment (ERGIC). However, it still remains elusive how cargo loading to vesicles, vesicle budding, tethering and fusion are coordinated in vivo. Here we show, using extremely high speed and high resolution confocal microscopy, that the cis-Golgi in the budding yeast Saccharomyces cerevisiae approaches and contacts the ERES. The COPII coat cage then collapses and the cis-Golgi captures cargo. The cis-Golgi, thus loaded with cargo, then leaves the ERES. We propose that this ‘hug-and-kiss’ behaviour of cis-Golgi ensures efficient and targeted cargo transport from the ERES to cis-Golgi.

Date: 2014
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DOI: 10.1038/ncomms4653

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