A red-shifted fluorescent substrate for aldehyde dehydrogenase

Il Minn, Haofan Wang, Ronnie C. Mease, Youngjoo Byun, Xing Yang, Julia Wang, Steven D. Leach and Martin G. Pomper ()
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Il Minn: Russel H. Morgan Department of Radiology and Radiological Science
Haofan Wang: Russel H. Morgan Department of Radiology and Radiological Science
Ronnie C. Mease: Russel H. Morgan Department of Radiology and Radiological Science
Youngjoo Byun: College of Pharmacy, Korea University, 2511 Sejong-ro, Jochiwon-eup, Sejong 339-700, South Korea
Xing Yang: Russel H. Morgan Department of Radiology and Radiological Science
Julia Wang: Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine
Steven D. Leach: Department of Surgery and the McKusick-Nathans Institute of Genetic Medicine
Martin G. Pomper: Russel H. Morgan Department of Radiology and Radiological Science

Nature Communications, 2014, vol. 5, issue 1, 1-9

Abstract: Abstract Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDHpos cells. We demonstrate that AldeRed 588-A successfully isolates ALDHhi human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDHpos cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDHpos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.

Date: 2014
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DOI: 10.1038/ncomms4662

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