Fabrication of enzyme-degradable and size-controlled protein nanowires using single particle nano-fabrication technique

Masaaki Omichi, Atsushi Asano, Satoshi Tsukuda, Katsuyoshi Takano, Masaki Sugimoto, Akinori Saeki, Daisuke Sakamaki, Akira Onoda, Takashi Hayashi and Shu Seki ()
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Masaaki Omichi: Graduate School of Engineering, Osaka University
Atsushi Asano: Graduate School of Engineering, Osaka University
Satoshi Tsukuda: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University
Katsuyoshi Takano: Graduate School of Engineering, Osaka University
Masaki Sugimoto: Japan Atomic Energy Agency
Akinori Saeki: Graduate School of Engineering, Osaka University
Daisuke Sakamaki: Graduate School of Engineering, Osaka University
Akira Onoda: Graduate School of Engineering, Osaka University
Takashi Hayashi: Graduate School of Engineering, Osaka University
Shu Seki: Graduate School of Engineering, Osaka University

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract Protein nanowires exhibiting specific biological activities hold promise for interacting with living cells and controlling and predicting biological responses such as apoptosis, endocytosis and cell adhesion. Here we report the result of the interaction of a single high-energy charged particle with protein molecules, giving size-controlled protein nanowires with an ultra-high aspect ratio of over 1,000. Degradation of the human serum albumin nanowires was examined using trypsin. The biotinylated human serum albumin nanowires bound avidin, demonstrating the high affinity of the nanowires. Human serum albumin–avidin hybrid nanowires were also fabricated from a solid state mixture and exhibited good mechanical strength in phosphate-buffered saline. The biotinylated human serum albumin nanowires can be transformed into nanowires exhibiting a biological function such as avidin–biotinyl interactions and peroxidase activity. The present technique is a versatile platform for functionalizing the surface of any protein molecule with an extremely large surface area.

Date: 2014
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DOI: 10.1038/ncomms4718

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