Targeted genomic rearrangements using CRISPR/Cas technology

Peter S. Choi and Matthew Meyerson ()
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Peter S. Choi: Dana-Farber Cancer Institute
Matthew Meyerson: Dana-Farber Cancer Institute

Nature Communications, 2014, vol. 5, issue 1, 1-6

Abstract: Abstract Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

Date: 2014
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DOI: 10.1038/ncomms4728

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