On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays

Yiqing Lu, Jie Lu, Jiangbo Zhao, Janet Cusido, Françisco M Raymo, Jingli Yuan, Sean Yang, Robert C. Leif, Yujing Huo, James A. Piper, J Paul Robinson, Ewa M. Goldys and Dayong Jin ()
Additional contact information
Yiqing Lu: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University
Jie Lu: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University
Jiangbo Zhao: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University
Janet Cusido: Laboratory for Molecular Photonics, University of Miami, 1301 Memorial Drive
Françisco M Raymo: Laboratory for Molecular Photonics, University of Miami, 1301 Memorial Drive
Jingli Yuan: State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology
Sean Yang: Newport Instruments, 3345 Hopi Place
Robert C. Leif: Newport Instruments, 3345 Hopi Place
Yujing Huo: Tsinghua University
James A. Piper: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University
J Paul Robinson: Purdue University Cytometry Laboratories, Bindley Bioscience Center, Purdue University
Ewa M. Goldys: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University
Dayong Jin: Advanced Cytometry Laboratories, ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP), Macquarie University

Nature Communications, 2014, vol. 5, issue 1, 1-8

Abstract: Abstract Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called ‘τ-Dots’. It provides a large dynamic range of lifetimes from microseconds to milliseconds, which allows creating large libraries of nanotags/microcarriers. However, a robust approach is required to rapidly and accurately measure the luminescence lifetimes from the relatively slow-decaying signals. Here we show a fast algorithm suitable for the microsecond region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique. We exploit this approach to further extend optical time-domain multiplexing to the downconversion luminescence, using luminescence microspheres wherein lifetimes are tuned through luminescence resonance energy transfer. We demonstrate real-time discrimination of these microspheres in the rapid scanning cytometry, and apply them to the multiplexed probing of pathogen DNA strands. Our results indicate that tunable luminescence lifetimes have considerable potential in high-throughput analytical sciences.

Date: 2014
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DOI: 10.1038/ncomms4741

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