Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range

Xiang Wu, Maung Kyaw Khaing Oo, Karthik Reddy, Qiushu Chen, Yuze Sun and Xudong Fan ()
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Xiang Wu: University of Michigan
Maung Kyaw Khaing Oo: University of Michigan
Karthik Reddy: University of Michigan
Qiushu Chen: University of Michigan
Yuze Sun: University of Texas at Arlington
Xudong Fan: University of Michigan

Nature Communications, 2014, vol. 5, issue 1, 1-7

Abstract: Abstract Enzyme-linked immunosorbent assay (ELISA) is a powerful method for biomolecular analysis. The traditional ELISA employing light intensity as the sensing signal often encounters large background arising from non-specific bindings, material autofluorescence and leakage of excitation light, which deteriorates its detection limit and dynamic range. Here we develop the optofluidic laser-based ELISA, where ELISA occurs inside a laser cavity. The laser onset time is used as the sensing signal, which is inversely proportional to the enzyme concentration and hence the analyte concentration inside the cavity. We first elucidate the principle of the optofluidic laser-based ELISA, and then characterize the optofluidic laser performance. Finally, we present the dual-mode detection of interleukin-6 using commercial ELISA kits, where the sensing signals are simultaneously obtained by the traditional and the optofluidic laser-based ELISA, showing a detection limit of 1 fg ml−1 (38 aM) and a dynamic range of 6 orders of magnitude.

Date: 2014
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DOI: 10.1038/ncomms4779

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