Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1

Saihong Jin, Jeehee Kim, Torsten Willert, Tanja Klein-Rodewald, Mario Garcia-Dominguez, Matias Mosqueira, Rainer Fink, Irene Esposito, Lorenz C. Hofbauer, Patrick Charnay and Matthias Kieslinger ()
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Saihong Jin: Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum München, National Research Center for Environmental Health
Jeehee Kim: Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum München, National Research Center for Environmental Health
Torsten Willert: Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum München, National Research Center for Environmental Health
Tanja Klein-Rodewald: Institute of Pathology, Helmholtz Zentrum München, National Research Center for Environmental Health
Mario Garcia-Dominguez: Developmental Biology Section, Ecole Normale Supérieure
Matias Mosqueira: Medical Biophysics Unit, Institute of Physiology and Pathophysiology, University of Heidelberg
Rainer Fink: Medical Biophysics Unit, Institute of Physiology and Pathophysiology, University of Heidelberg
Irene Esposito: Institute of Pathology, Helmholtz Zentrum München, National Research Center for Environmental Health
Lorenz C. Hofbauer: Diabetes and Metabolic Bone Diseases, TU Dresden Medical Center
Patrick Charnay: Developmental Biology Section, Ecole Normale Supérieure
Matthias Kieslinger: Institute of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum München, National Research Center for Environmental Health

Nature Communications, 2014, vol. 5, issue 1, 1-16

Abstract: Abstract Myogenic regulatory factors such as MyoD and Myf5 lie at the core of vertebrate muscle differentiation. However, E-boxes, the cognate binding sites for these transcription factors, are not restricted to the promoters/enhancers of muscle cell-specific genes. Thus, the specificity in myogenic transcription is poorly defined. Here we describe the transcription factor Ebf3 as a new determinant of muscle cell-specific transcription. In the absence of Ebf3 the lung does not unfold at birth, resulting in respiratory failure and perinatal death. This is due to a hypercontractile diaphragm with impaired Ca2+ efflux-related muscle functions. Expression of the Ca2+ pump Serca1 (Atp2a1) is downregulated in the absence of Ebf3, and its transgenic expression rescues this phenotype. Ebf3 binds directly to the promoter of Atp2a1 and synergises with MyoD in the induction of Atp2a1. In skeletal muscle, the homologous family member Ebf1 is strongly expressed and together with MyoD induces Atp2a1. Thus, Ebf3 is a new regulator of terminal muscle differentiation in the diaphragm, and Ebf factors cooperate with MyoD in the induction of muscle-specific genes.

Date: 2014
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DOI: 10.1038/ncomms4793

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