Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

Marcus Gallagher-Jones, Yoshitaka Bessho, Sunam Kim, Jaehyun Park, Sangsoo Kim, Daewoong Nam, Chan Kim, Yoonhee Kim, Do Young Noh, Osamu Miyashita, Florence Tama, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Kensuke Tono, Yoshiki Kohmura, Makina Yabashi, S. Samar Hasnain, Tetsuya Ishikawa and Changyong Song ()
Additional contact information
Marcus Gallagher-Jones: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Yoshitaka Bessho: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Sunam Kim: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Jaehyun Park: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Sangsoo Kim: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Daewoong Nam: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Chan Kim: Gwangju Institute of Science and Technology
Yoonhee Kim: Gwangju Institute of Science and Technology
Do Young Noh: Gwangju Institute of Science and Technology
Osamu Miyashita: RIKEN Advanced Institute for Computational Science, 7-1-26 Minatojima-Minamimach, Chuo-ku, Kobe 650-0047, Japan
Florence Tama: RIKEN Advanced Institute for Computational Science, 7-1-26 Minatojima-Minamimach, Chuo-ku, Kobe 650-0047, Japan
Yasumasa Joti: Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan
Takashi Kameshima: Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan
Takaki Hatsui: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Kensuke Tono: Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan
Yoshiki Kohmura: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Makina Yabashi: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
S. Samar Hasnain: Institute of Integrative Biology, University of Liverpool
Tetsuya Ishikawa: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan
Changyong Song: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan

Nature Communications, 2014, vol. 5, issue 1, 1-9

Abstract: Abstract Nanostructures formed from biological macromolecular complexes utilizing the self-assembly properties of smaller building blocks such as DNA and RNA hold promise for many applications, including sensing and drug delivery. New tools are required for their structural characterization. Intense, femtosecond X-ray pulses from X-ray free-electron lasers enable single-shot imaging allowing for instantaneous views of nanostructures at ambient temperatures. When combined judiciously with synchrotron X-rays of a complimentary nature, suitable for observing steady-state features, it is possible to perform ab initio structural investigation. Here we demonstrate a successful combination of femtosecond X-ray single-shot diffraction with an X-ray free-electron laser and coherent diffraction imaging with synchrotron X-rays to provide an insight into the nanostructure formation of a biological macromolecular complex: RNA interference microsponges. This newly introduced multimodal analysis with coherent X-rays can be applied to unveil nano-scale structural motifs from functional nanomaterials or biological nanocomplexes, without requiring a priori knowledge.

Date: 2014
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DOI: 10.1038/ncomms4798

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